Abstract： AS 4276.3:2021 pdf download.Water microbiology Method 3: Enumeration of heterotrophic microorganisms—Pour plate,spread plate, membrane filtration and most probable number techniques. 6.3 Reference cultures 6.3.1 General The reference cult...

AS 4276.3:2021 pdf download.Water microbiology Method 3: Enumeration of heterotrophic microorganisms—Pour plate,spread plate,

membrane filtration and most probable number techniques.

6.3 Reference cultures

6.3.1 General

The reference cultures are used in media quality control and analytical quality control procedures relevant to this method. The

purpose of the reference cultures is to demonstrate and ensure that typical growth characteristics are exhibited by the cultures on the media used.

As a minimum, the reference cultures shall be used to ensure that the method has been used properly. Each day that samples are

processed by this method, suspensions of the reference cultures shall be submitted to the test procedure.

If the reference cultures do not give appropriate results, then an investigation shall be undertaken. Test results shall be invalid unless the investigation reveals reasons for this failure that invalidate the quality control procedures (e.g. prepared suspensions shown not to contain target bacteria).

6.3.2 Positive culture

Escherichia coil, WDCM 00090, WDCM 00012 or WDCM 00013 or a culture traceable to any of these strains.

NOTE World Data Centre for Microorganisms (WDCM).

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