Abstract： AS 5013.14.2:2009 pdf download.Food microbiology Method 14.2: General procedures and techniques-Colony count-Membrane filtration method. 5 APPARATUS The following apparatus is required: (a) Membrane filtration apparatus. NOTE: Various types...

AS 5013.14.2:2009 pdf download.Food microbiology Method 14.2: General procedures and techniques-Colony count-Membrane filtration

method.

5 APPARATUS

The following apparatus is required:

(a) Membrane filtration apparatus.

NOTE: Various types of apparatus suitable tór the membrane liltration method are aailahIe commercially in Australia.

(b) Grid-mark membrane filters of 47 mm to 50 mm diameter to fit the apparatus and having a pore size appropriate to the organism

or organisms to be assessed. The usual size for water is 0.45 tm.

NOTE: Sterile membrane fillers are commercially available.

(C) A filter flask of capacity appropriate to the volume of liquid lobe filtered.

(d) A Source of vacuum.

(e) Forceps that will not damage the membrane.

(f) Petri dishes.

(g) Graduated measuring cylinders, where required, suitable for measuring the volume of sample to be filtered.

(h) Graduated pipettes.

(i) A tally counter.

A suitable incubator.

(k) Non-toxic absorbent filter pads of diameter either equal to or slightly greater than the diameter of the membrane filters with which they are to he used.

(I) Pasteur pipettes.

NOTE: Items (k) and (I) above are only required where the nutrient pad technique is to be used.

Recommended：