Abstract： AS 5013.18:2010 pdf download.Food microbiology Method 18: Examination for specific organisms—Vibrio parahaemolyticus. 6 TEST PROCEDURES The procedure shall be as follows: (a) Using the most probable number (MPN) method (3-tube technique)...
AS 5013.18:2010 pdf download.Food microbiology Method 18: Examination for specific organisms—Vibrio parahaemolyticus.
6 TEST PROCEDURES
The procedure shall be as follows:
(a) Using the most probable number (MPN) method (3-tube technique) described in AS 5013.3. inoculate tubes containing 10 ml. of alkaline peptone water (4.2.1) with appropriate dilutions of the test sample (primary enrichment).
(b) Prepare a set of controls for each series of tests by inoculating tubes of alkaline peptone water (4.2. I) with the reference
culture(s) specitied in Clause 4.5.
(c) Incubate tests and control at 36 ±2°C for 6 h to 8 h.
(d) Transfer I mL from each tube to a fresh tube of alkaline peptone water (4.2.1) (secondary enrichment). Streak a loopful of each primary enrichment culture on to a prepared plate of TCBS agar (4.2.2) to obtain isolated colonies.
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