Abstract： AS 5013.3:2022 pdf download.Food microbiology Method 3: Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of coliforms - Most probablenumber technique (ISO 4831:2006, MOD). Fnumeration is...
AS 5013.3:2022 pdf download.Food microbiology Method 3: Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of coliforms - Most probablenumber technique (ISO 4831:2006, MOD).
Fnumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or
37 °C.
NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the temperature of incubation is 30 °C.
This enumeration method is applicable when the number sought is expected to be in the range ito 100 per millilitre or per gram of
test sample.
A limitation on the applicability of this International Standard is imposed by the susceptibility of the method to a large degree
of variability. The information given in Clause 11 provides guidance on the applicability of the method and on the interpretation
of the results.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition
cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 6887 (all parts), Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
ISO 8261, Milk and milk products — General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination
ISO/TS 11 133-1, Microbiology offood and animal feeding stuffs — Guidelines on preparation and production of culture media —
Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory.
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