Abstract： AS 4276.17.2:2016 pdf download.Water microbiology Method 17.2: Spores of Clostridium perfringens—Estimation of most probablenumber (MPN) using the multiple tube dilution technique. 5.2.3 Uce of reference cultures The reference cultures ar...

AS 4276.17.2:2016 pdf download.Water microbiology Method 17.2: Spores of Clostridium perfringens—Estimation of most probablenumber (MPN) using the multiple tube dilution technique.

5.2.3 Uce of reference cultures

The reference cultures are used in media quality control and analytical quality control procedures relevant to this method. The

purpose of the reference cultures is to demonstrate and ensure that typical growth characteristics and test reactions are exhibited by the cultures on the media and in the tests used in this method.

When testing a sample of water by this standard method, a culture of the positive reference microorganism (5.2.1) shall be

submitted to the test procedures at the same time to demonstrate and ensure that typical growth characteristics and test reactions are exhibited. The negative reference microoganism (5.2.2) is used in addition to the positive reference microorganism for the

acid phosphatase test.

If the reference cultures do not give appropriate results, then an investigation shall he undertaken. Test results shall be

regarded as invalid unless the investigation reveals reasons that invalidate the quality control procedures, e.g. prepared

suspension shown not to contain target bacteria.

Recommended：